1.
Intracellular pH vital cells
by fluorescence emission ratioing
esterase substrate: 1,4-diaceroxy-2,3-dicyanobenzene (ADB)
intracellular pH indicator: dicyanohydroquinone = phthalonitrile (DCH)
DNA dead cells: propidium iodide (PI)
1.1 Photograph:
rat bone marrow cells (ADB/PI)
1.2 3D data display:
human blood leukocytes (ADB/PI)
1.3 Further references 1.G Valet, A Raffael, L Moroder, E Wünsch, G Ruhenstroth-Bauer.
Fast intracellular pH determination in single cells by flow- cytometry.
Naturwissenschaften (1981) 68: 265-266
2.G Valet, A Raffael.
Cyto-P-Check: Determination of intracellular pH and esterase activity
in vital cells by flow-cytometry.
Paesel, Frankfurt (1984) p 1-21.
3.G Valet, HH Warnecke, H Kahle.
Cyto-P-Check: Cytostatic drug testing on patient tumor cells.
Paesel GmbH, Frankfurt, (1984) p 22-30.
4.G Rothe, G Valet.
Phagocytosis, intracellular pH, and cell volume in the multifunctional
analysis of granulocytes by flow-cytometry.
Cytometry (1988) 9: 316-324.
5.G Rothe, W Kellermann, G Valet.
Flow cytometric analysis of phagocytosis, respiratory burst,
intracellular pH and cytosolic free calcium of granulocytes of
posttraumatic and septic patients.
In: Immune Consequences of Trauma, Shock and Sepsis,
Eds: E Faist, J Ninnemann, D Green, Springer Verlag, Berlin (1989),
p 235- 240.
6.G Rothe, W Kellermann, G Valet.
Flow cytometric parameters of neutrophil function as early indicators
of sepsis- or trauma-related pulmonary or cardiovascular organ failure.
J Lab Clin Invest (1990) 115:52-61.
7.G Rothe, W Kellermann, J Briegel, B Schaerer, G Valet.
Activation of neutrophils by tumor necrosis factor-a during sepsis.
In: Immune Consequences of Trauma, Shock and Sepsis Vol.II,
Eds: E Faist, J Ninnemann, D Green, Springer Verlag, Berlin (1993),
p 727-733.
2. 3D data display 1.G Valet.
Graphical respresentation of three parameter flow cytometer histograms
by a newly developed FORTRAN IV computer program.
In: Flow Cytometry IV, Eds: OD Laerum T Lindmo, E Thorud,
Universitetsforlaget, Oslo (1980), p 125-129.
