Durchflusszytometrie: Intrazellulärer pH Wert und 3D Datendarstellung

former: Arbeitsgruppe Zellbiochemie
Max-Planck-Institut für Biochemie, Martinsried

1. Intrazellulärer pH Wert (Messung Fluoreszenzemissionsverhältnis)
      Esterasesubstrat: 1,4-diaceroxy-2,3-dicyanobenzol (ADB)
      Intrazellulärer pH Wert Indikator: Dicyanohydrochinon = Phthalonitril (DCH)
      DNA tote Zellen: Propidium Iodid (PI)
1.1 Foto: Rattenknochenmarkszellen (ADB/PI)
1.2 3D Datendarstellung: menschliche Blutleukozyten (ADB/PI)
1.3 Weitere Referenzen
1. G Valet, A Raffael, L Moroder, E Wünsch, G Ruhenstroth-Bauer. Fast intracellular pH determination in single cells by flow- cytometry. Naturwissenschaften (1981) 68: 265-266
2. G Valet, A Raffael. Cyto-P-Check: Determination of intracellular pH and esterase activity in vital cells by flow-cytometry. Paesel, Frankfurt (1984) p 1-21.
3. G Valet, HH Warnecke, H Kahle. Cyto-P-Check: Cytostatic drug testing on patient tumor cells. Paesel GmbH, Frankfurt, (1984) p 22-30.
4. G Rothe, G Valet. Phagocytosis, intracellular pH, and cell volume in the multifunctional analysis of granulocytes by flow-cytometry. Cytometry (1988) 9: 316-324.
5. G Rothe, W Kellermann, G Valet. Flow cytometric analysis of phagocytosis, respiratory burst, intracellular pH and cytosolic free calcium of granulocytes of posttraumatic and septic patients. In: Immune Consequences of Trauma, Shock and Sepsis, Eds: E Faist, J Ninnemann, D Green, Springer Verlag, Berlin (1989), p 235- 240.
6. G Rothe, W Kellermann, G Valet. Flow cytometric parameters of neutrophil function as early indicators of sepsis- or trauma-related pulmonary or cardiovascular organ failure. J Lab Clin Invest (1990) 115:52-61.
7. G Rothe, W Kellermann, J Briegel, B Schaerer, G Valet. Activation of neutrophils by tumor necrosis factor-a during sepsis. In: Immune Consequences of Trauma, Shock and Sepsis Vol.II, Eds: E Faist, J Ninnemann, D Green, Springer Verlag, Berlin (1993), p 727-733.

2. 3D Datendarstellung
1. G Valet. Graphical respresentation of three parameter flow cytometer histograms by a newly developed FORTRAN IV computer program. In: Flow Cytometry IV, Eds: OD Laerum T Lindmo, E Thorud, Universitetsforlaget, Oslo (1980), p 125-129.

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last update: Nov 01,2025
first display: Oct 29,2025