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Esterases/Phosphatases
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HR Hulett, WA Bonner, J Barrett, LA Herzenberg
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Cell sorting - Automated separation of mammalian cells as a function
of intracellular fluorescence. Science 1969, 166: 747-749.
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G von Sengbusch, C Couwenbergs, J Kühner, U Müller
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Fluorogenic substrates in single living cells.
Histochem J 1976, 8: 341-350.
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ME Wilder, LS Cram
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Differential fluorochromasia of human lymphocytes as measured
by flow cytometry.
J Histochem Cytochem 1977, 25: 888-891.
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JV Watson, SH Chambers, P Workman, TS Horsnell
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Flow cytofluorimetric method for measuring enzyme reaction
kinetics in intact cells. FEBS letters 1977, 81: 179-182.
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JV Watson
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Enzyme kinetic-studies in cell-populations using fluorogenic
substrates and flow cytometric techniques. Cytometry 1980, 1:143-151.
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J Malin-Berdel, G Valet
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Flow cytometric determination of esterases and phosphatase
activities and kinetics in hematopoietic cells with
fluorogenic substrates. Cytometry 1980, 1: 222-228
(41).
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Beta-glucuronidase
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FA Dolbeare, WF Phares
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Napthol AS-BI(7-bromo-3-hydroxy-2-naphtho-o-anisidine)
phosphatase and naphtol AS-BI beta-d-glucuronidase in
chinese-hamster ovary cells - Biochemical and flow
cytometric studies.
J Histochem Cytochem 1979, 27: 120-124.
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Surface charge density (FITC-polyornithine, polylysine)
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G Valet, S Bamberger G Ruhenstroth-Bauer
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Flow cytometric determination of surface charge density
of the erythrocyte membrane using fluorescinated
polycations.
J Histochem Cytochem 1979, 27: 342-349
(31).
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Vital and dead cell discrimination (Hoechst 33662/PI)
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M Stöhr, M Vogt-Schaden
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A dual staining technique for simultaneous flow cytometric DNA analysis
of living and dead cells.
In: Flow Cytometry IV,
Eds: OD Laerum, T Lindmo, E Thorud, Universitetsforlaget,
Bergen 1980 p 96-99.
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Intracellular pH (fluorescein diacetate (FDA))
double excitation fluorescence ratios (cell cluster pH by two measurements)
|
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JWM Visser, AAM Jongeling, HJ Tanke
|
Intracellular pH determination by fluorescence measurements.
J Histochem Cytochem 1979, 27: 32-35.
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Intracellular pH (di-cyanohydrochinone (DCH))
fluorecence emission ratios (single cell pH)
|
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G Valet, A Raffael, L Mororder, E Wünsch, G Ruhenstroth-Bauer
|
Fast intracellular pH determination in single cells by flow-cytometry.
Naturwiss 1981, 68: 265-266
(44).
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G Valet, G Ruhenstroth-Bauer, E Wünsch, L Moroder
|
Process of determining the pH value in the interior of a cell
US Patent 4,677,060 priority Feb 6, 1981 (DE)
|
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G Valet, G Ruhenstroth-Bauer, E Wünsch, L Moroder
|
Compounds for determining the pH value in the interior of a cell.
US Patent 4,515,730 priority Feb 6, 1981 (DE)
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Phagocytosis
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CD Bassoe, J Solsvik, OD Laerum
|
Quantitation of single cell phagocytic capacity by flow cytometry.
In: Flow Cytometry IV,
Eds: OD Laerum, T Lindmo, E Thorud,
Universitetsforlaget, Bergen 1980 p 170-174.
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Intracellular Ca2+ determination (INDO1)
|
|
G Valet, A Raffael
|
Determination of intracellular calcium in vital cells by flow cytometry.
Naturwiss 1985, 72: 600-602
(66).
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Respiratory burst 2',7'-dichlorofluorescin (DCFH)
|
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DA Bass, P Olbrantz, P Szejda, MC Seeds, CE McCall
|
Subpopulations of neutrophils with increased oxidative
product formation by neutrophils: A graded response to
membrane stimulation.
J Immunol 1983, 130: 1910-1917.
|
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Respiratory burst dihydrorhodamine123 (DHR123)
|
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G Rothe, A Oser, G Valet
|
Dihydrorhodamine123: A new flow cytometric indicator for
respiratory burst activity.
Naturwiss 1988, 75: 354-355
(87).
|
|
Respiratory burst O2-indicator hydroethidine (HE)
|
|
G Rothe, G Valet
|
Flow cytometric analysis of respiratory burst activity in
phagocytes with hydroethidine and 2'7'-dichlorofluorescin.
J Leu Biol 1990, 47: 440-448
(108).
|
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Glutathione (orthophtaldialdehyde(OPT))
|
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J Treumer, G Valet
|
Flow-cytometric determination of glutathione alterations in
vital cells by o-phtahldialdehyde.
Exp Cell Res 1986, 163: 518-524
(72).
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Glutathione (monobromobimane with N-ethylmaleimide protein thiol group blocking)
|
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M Poot, A Verkerk, JF Koster, JF Jongkind
|
De novo synthesis of glutathione in human fibroblasts during
in-vitro aging and some metabolic diseases as measured by
a flow cytometric method
BBA 1986, 883: 580-584.
|
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Intracellular proteinase activites (non viable cells)
|
|
FA Dolbeare, RE Smith
|
Flow cytometric measurement of peptidases with use of 5-nitrosalicylaldehyde
and 4-methoxy-beta-naphthylamine.
J Clin Chem 1977, 23: 1485-1491.
|
|
Intracellular proteinase activites (R110-substrates in viable cells)
|
|
G Rothe, I Assfalg-Machleidt, W Machleidt, G Valet
|
Independent regulation of endopeptidase activity and
respiratory burst activity of neutrophils analyzed by
flow cytometry.
In: Advances in analytical cellular Pathology,
Eds: G Burger, M Oberholzer, GP Voijs,
Excerpta Medica, Amsterdam 1990 p 119-120
(101).
|
|
I Assfalg-Machleidt, G Rothe, S Klingel, R Banati, WF Mangel,
G Valet, W Machleidt
|
Membrane permeable fluorogenic rhodamine substrates for
selective determination of cathepsin L.
Biol Chem Hoppe Seyler 1992, 373: 433-440
(121).
|
|
G Rothe, S Klingel, I Assfalg-Machleidt, W Machleidt, C Zirkelbach,
RB Banati, WF Mangel, G Valet.
|
Flow cytometric analysis of protease activities in vital cells.
Biol Chem Hoppe Seyler 1992, 373: 547-554
(120).
|
|
S Klingel, G Rothe, W Kellermann, G Valet
|
Flow cytometric determination of cysteine and serine proteinase
activities in living cells with rhodamine110 substrates.
Methods Cell Biol 1994, 41: 449-459
(145).
|
|
S Ganesh, S Klingel, H Kahle, G Valet
|
Flow cytometric determination of aminopeptidase activities in
viable cells using fluorogenic rhodamine110 substrates.
Cytometry 1995, 20: 334-340
(148).
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