Concepts in Cytomics G.Valet former: Cell Biochemistry Group Max-Planck-Institut für Biochemie, Martinsried

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The goal of this concept display is to advance the scientific discussion on the potential of cytomics in basic as well as in clinical research with regard to predictive medicine, biomolecular diagnostics and consensus development at an interdisciplinary level.
- Disease related processes can be determined at the cellular level by cytometric methods since diseases are caused by molecular changes in cellular systems or organs. This permits disease detection (diagnosis) as well as the prediction on future disease progression in individual patients.
- Clinical cytometry addresses frequently immunological and hematological issues by cellular immunophenotyping as major methodology e.g. in leukemia classification, hematopoietic stem cell or kidney transplantation and HIV infection monitoring.
- Multiparameter measurements of cell functions in conjunction with antibody binding and DNA determinations are important for the detection and follow-up of fast developing clinical processes like infectious, allergic, thrombotic or rheumatoid affections as well as for the control of therapy.

1. General Concepts

   Cytome & Cytomics (definition)
   Cytomics, a Biomedical Key Discipline
   Predictive Medicine by Cytomics
   Human cytome project (Günter Valet, Attila Tarnok)
     (2003, 2004a, 2004b, 2005, PDF, definitions, references)
   Human Cytome Project (2003a/2003b Peter van Osta)
   Data Mining & Knowledge Extraction in Cytomics
   Data Pattern Classification versus Modelling
      = Low complexity: Modelling for data reduction
          (mean volume, coefficient of variation (CV), % frequency
          of erythrocyte populations)
      - erythrocyte volume populations (1-param Gaussians)
         analytical (3, 2, 1)
         preparative (6, 5, 4)
      - erythrocyte volume populations (2-param Gaussians)
         analytical (7)
      = High complexity: Data pattern classification
         (standardized 5-15 parameter disease & patient classification masks)
       - predictive medicine by cytomics
   CyTOF Data Analysis: Discrimination or Correlation ?
   20 years DGfZ (PDF)
   30 years DGfZ (PDF)

     Tissues & Cells
   Human Toponome Project (Walter Schubert)
   Toponomics (Martin Fraunholz)
   Tissomics (Attila Tarnok)
   Tissomics: Cytomics goes 3D (Rupert Ecker, Attila Tarnok)
   Cytometry in Cell Biology (Zbigniew Darzynkiewicz)
   Microorgamisms and Plant Cells

     Cytometry History
   Cytomics & Flow Cytometry at Martinsried
   Analytical Cellular Pathology: Presence and Future
   Early Concepts in Flow Cytometry

2. Clinical Cytomics

   Cytomics, from Prognostic to Predictive Medicine
   Cytomics, a Practical Approach
   Automated Disease Classification
   Cellular Diagnosis in Vascular Medicine

3. Organisational Concepts

   European Working Group on Clinical Cell Analysis (EWGCCA)
   Europ.Fed.of Cytometric Societies

• < Cell Biochemistry    < CytoRelay

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